Qiagen Pcr Purification Kit


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Hi, I'm new, and I posted this question up in the "pinned" topics, but maybe I should be starting a new topic for this? I don't know. Here's my question:

"This is a silly question, but I was just thinking today about how I use the Qiagen PCR purification kit all the time, but have no idea how it works! What do the buffers PB and PE do? I guess PB helps DNA bind to the spin column, but how? And why do you need to add ethanol to buffer PE? What does it do? Thanks so much."



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