Qiagen Ffpe

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The RNeasy FFPE Kit is specially designed for purifying total RNA from formalin-fixed, paraffin-embedded tissue sections. Special lysis and incubation conditions reverse formaldehye modification of RNA. In addition, the lysis buffer efficiently releases RNA from tissue sections while avoiding further RNA degradation. The kit also uses DNase and DNase Booster Buffer for optimized removal of genomic DNA contamination. RNeasy MinElute spin columns enable purification of total RNA with elution volumes of as low as 10 μl. Purification can be fully automated on the  QIAcube .

Genomic DNA was purified from different FFPE tissue samples using the QIAamp DNA FFPE Tissue Kit. Products of the prnp gene (78 bp, 464 bp, and 727 bp) were amplified by real-time quantitative PCR.

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